王茹茹;李启霖;田卫卫;王鑫雨;姜兴岳;

• 1:滨州医学院附属医院放射科

摘要(Abstract)：

酰胺质子转移(amide proton transfer,APT)成像是化学交换饱和转移(chemical exchangesaturationtransfer,CEST)成像中发展较为迅速和成熟的一种,它以磁化传递和化学交换理论为基础,通过探测人体内游离蛋白质和多肽链上的酰胺质子与水中氢离子的交换速率来评估细胞内蛋白质浓度和pH值的变化情况。APT成像作为一种无创的细胞分子水平磁共振技术,能够在脑损伤、脑肿瘤、神经退行性疾病及儿童脑发育过程中提供重要的影像学信息,为临床的诊断、治疗及预后方面提供帮助。本研究主要阐述APT成像在中枢神经系统中的应用现状及进展。

关键词(KeyWords)： 酰胺质子转移;磁共振成像;蛋白质;酸碱度;中枢神经系统

Abstract:

Keywords:

基金项目(Foundation): 山东省自然科学基金(编号：ZR2018LH015)~~

作者(Author): 王茹茹;李启霖;田卫卫;王鑫雨;姜兴岳;

Email:

DOI:

参考文献(References)：

• [1]Zhou J,Lal B,Wilson DA,et al.Amide proton transfer(APT) contrast for imaging of brain tumors.Magn Reson Med,2003,50(6):1120-1126.DOI:10.1002/mrm.10651.
• [2]Van Zijl PC,Zhou J,Mori N,et al.Mechanism of magnetization transfer during on-resonance water saturation.A new approach to detect mobile proteins,peptides,and lipids.Magn Reson Med,2003,49(3):440-449.DOI:10.1002/mrm.10398.
• [3]Van Zijl PC,Yadav NN.Chemical exchange saturation transfer(CEST):what is in a name and what isn't?.Magn Reson Med,2011,65(4):927-948.DOI:10.1002/mrm.22761.
• [4]Vinogradov E,Sherry AD,Lenkinski RE.CEST:from basic principles to applications,challenges and opportunities.J Magn Reson,2013,229(4):155-172.DOI:10.1016/j.jmr.2012.11.024.
• [5]Zheng Y,Wang X,Zhao X.Magnetization transfer and amide proton transfer MRI of neonatal brain development.Biomed Res Int,2016,2016:3052723.DOI:10.1155/2016/3052723.
• [6]Zhang H,Kang H,Zhao X,et al.Amide proton transfer(APT) MRimaging and magnetization transfer(MT) MR imaging of pediatric brain development.Eur Radiol,2016,26(10):3368-3376.DOI:10.1007/s00330-015-4188-z.
• [7]Zhang Z,Zhang C,Yao J,et al.Amide proton transfer-weighted magnetic resonance imaging of human brain aging at 3 Tesla.Quant Imaging Med Surg,2020,10(3):727-742.DOI:10.21037/qims.2020.02.22.
• [8]Zheng Y,Wang XM.Amide proton transfer imaging of neonatal piglet with hypoxic ischemic brain injury.Chin J Med Imaging Technol,2017,33(8):1153-1157.DOI:10.13929/j.1003-3289.201611080.郑阳,王晓明.新生猪缺氧缺血脑损伤后酰胺质子转移成像.中国医学影像技术,2017,33(8):1153-1157.DOI:10.13929/j.1003-3289.201611080.
• [9]Zheng Y,Wang XM.Evaluation of brain injury in neonates by magnetization transfer imaging combined amide proton transfer imaging:a preliminary study.Chin J Magn Reson Imaging,2017,8(3):189-195.DOI:10.12015/issn.1674-8034.2017.03.006.郑阳,王晓明.磁化传递成像和酰胺质子转移成像联合评价新生儿脑损伤的初步研究.磁共振成像,2017,8(3):189-195.DOI:10.12015/issn.1674-8034.2017.03.006.
• [10]Chen SJ.Diagnostic value of amide proton transfer magnetic resonance imaging in neonatal hypoxic-ischemic encephalopathy.Guangzhou:Southern Medical University,2019.陈思瑾.磁共振酰胺质子转移成像在新生儿缺血缺氧性脑损伤诊断中的应用价值.广州:南方医科大学,2019.
• [11]Sun PZ,Zhou J,Sun W,et al.Detection of the ischemic penumbra using pH-weighted MRI.J Cereb Blood Flow Metab,2007,27(6):1129-1136.DOI:10.1038/sj.jcbfm.9600424.
• [12]Huang D,Li S,Dai Z,et al.Novel gradient echo sequence based amide proton transfer magnetic resonance imaging in hyperacute cerebral infarction.Mol Med Rep,2015,11(5):3279-3284.DOI:10.3892/mmr.2015.3165.
• [13]Zhang S,Li CM,Song GD,et al.Application of amide proton transfer magnetic resonance imaging in staging of ischemic cerebral infarction.Shandong Med J,2016,56(43):52-54.DOI:10.3969/j.issn.1002-266X.2016.43.016.张帅,李春媚,宋国栋,等.酰胺质子转移磁共振成像在缺血性脑梗死分期中的应用.山东医药,2016,56(43):52-54.DOI:10.3969/j.issn.1002-266X.2016.43.016.
• [14]Yu L,Chen Y,Chen M,et al.Amide proton transfer MRI signal as a surrogate biomarker of ischemic stroke recovery in patients with supportive treatment.Front Neurol,2019,10:104.DOI:10.3389/fneur.2019.00104.
• [15]Sun PZ,Cheung JS,Wang E,et al.Association between pH-weighted endogenous amide proton chemical exchange saturation transfer MRIand tissue lactic acidosis during acute ischemic stroke.J Cereb Blood Flow Metab,2011,31(8):1743-1750.DOI:10.1038/jcbfm.2011.23.
• [16]Zhou J,van Zijl PC.Defining an acidosis-based ischemic penumbra from pH-weighted MRI.Transl Stroke Res,2011,3(1):76-83.DOI:10.1007/s12975-011-0110-4.
• [17]Harston GW,Tee YK,Blockley N,et al.Identifying the ischaemic penumbra using pH-weighted magnetic resonance imaging.Brain,2015,138(Pt 1):36-42.DOI:10.1093/brain/awu374.
• [18]Tan YF.Use of rotating frame(T1ρ) and apparent diffusion coefficient(ADC) obtained from MRI with TI relaxation time to estimate cerebral ischemia evolution preliminary research of ischemic penumbra in subacute stroke patients combinating use of amide proton transfer(APT) with arterial spin-labeling(ASL).Guangzhou:Southern Medical University,2018.谭月发.旋转坐标系下时间常数(T1ρ)和表观扩散系数(ADC)在评估缺血性脑卒中演变进程中的对比应用&酰胺质子转移成像(APT)和动脉自旋标记(ASL)联合评价亚急性脑梗中缺血半暗带的初步研究.广州:南方医科大学,2018.
• [19]Yan K,Fu Z,Yang C,et al.Assessing amide proton transfer(APT) MRIcontrast origins in 9 L gliosarcoma in the rat brain using proteomic analysis.Mol Imaging Biol,2015,17(4):479-487.DOI:10.1007/s11307-015-0828-6.
• [20]Debnath A,Gupta RK,Singh A.Evaluating the role of amide proton transfer(APT)-weighted contrast,optimized for normalization and region of interest selection,in differentiation of neoplastic and infective mass lesions on 3 T MRI.Mol Imaging Biol,2020,22(2):384-396.DOI:10.1007/s11307-019-01382-x.
• [21]Chen Y,Li X,Song Y,et al.The diagnostic efficacy of amide proton transfer imaging in grading gliomas and predicting tumor proliferation.Neuroreport,2019,30(2):139-144.DOI:10.1097/WNR.0000000000001174.
• [22]Joo B,Han K,Ahn SS,et al.Amide proton transfer imaging might predict survival and IDH mutation status in high-grade glioma.Eur Radiol,2019,29(12):6643-6652.DOI:10.1007/s00330-019-06203-x.
• [23]Jiang S,Eberhart CG,Lim M,et al.Identifying recurrent malignant glioma after treatment using amide proton transfer-weighted MRimaging:A validation study with image-guided stereotactic biopsy.Clin Cancer Res,2019,25(2):552-561.DOI:10.1158/1078-0432.CCR-18-1233.
• [24]Jiang S,Yu H,Wang X,et al.Molecular MRI differentiation between primary central nervous system lymphomas and high-grade gliomas using endogenous protein-based amide proton transfer MR imaging at 3Tesla.Eur Radiol,2016,26(1):64-71.DOI:10.1007/s00330-015-3805-1.
• [25]Yu H,Lou H,Zou T,et al.Applying protein-based amide proton transfer MR imaging to distinguish solitary brain metastases from glioblastoma.Eur Radiol,2017,27(11):4516-4524.DOI:10.1007/s00330-017-4867-z.
• [26]Kamimura K,Nakajo M,Yoneyama T,et al.Histogram analysis of amide proton transfer-weighted imaging:comparison of glioblastoma and solitary brain metastasis in enhancing tumors and peritumoral regions.Eur Radiol,2019,29(8):4133-4140.DOI:10.1007/s00330-018-5832-1.
• [27]Chen L,Wang KP,Sun XP,et al.Analysis of the value of PWI in differential diagnosis of single-brain metastatic tumors from high-grade gliomas.J Med Imaging,2018,28(12):1966-1970.陈莉,王开平,孙先普,等.PWI鉴别诊断单发脑转移瘤和高级别胶质瘤的价值分析.医学影像学杂志,2018,28(12):1966-1970.
• [28]Li C,Peng S,Wang R,et al.Chemical exchange saturation transfer MRimaging of Parkinson's disease at 3 Tesla.Eur Radiol,2014,24(10):2631-2639.DOI:10.1007/s00330-014-3241-7.
• [29]Wang R,Ban YQ,Li CM,et al.The correlation between brain MRamide proton imaging and scores of clinical psychometric scales in patients with Alzheimer's disease.Chin J Magn Reson Imaging,2018,9(3):161-165.DOI:10.12015/issn.1674-8034.2018.03.001.王蕊,班允清,李春媚,等.阿尔茨海默病脑磁共振氨基质子转移成像与临床心理量表评分的相关性研究.磁共振成像,2018,9(3):161-165.DOI:10.12015/issn.1674-8034.2018.03.001.
• [30]Wang R,Chen J,Li CM,et al.A preliminary study on amide proton transfer MR imaging of Alzheimer’s disease and mild cognitive impairment in elder patients.Chin J Geriatrics,2015,34(10):1080-1083.DOI:10.3760/cma.j.issn.0254-9026.2015.10.008.王蕊,陈涓,李春媚,等.阿尔茨海默病和轻度认知功能障碍患者的磁共振酰胺质子转移成像初步研究.中华老年医学杂志,2015,34(10):1080-1083.DOI:10.3760/cma.j.issn.0254-9026.2015.10.008.
• [31]Wang R,Chen P,Shen Z,et al.Brain amide proton transfer imaging of rat with Alzheimer's disease using saturation with frequency alternating RF irradiation method.Front Aging Neurosci,2019,11:217.DOI:10.3389/fnagi.2019.00217.