邢培秋;陈秋雁;吴富淋;彭晓澜;江敏;陈婷婷;魏鼎泰;

• 1:福建医科大学附属宁德市医院放射科

摘要(Abstract)：

目的探讨缺血性脑卒中大鼠模型在水通道蛋白磁共振分子成像(AQP MRI)上的改变。材料与方法 31只成年雄性SD大鼠随机分为两组,实验组和假手术组。实验组经历1 h的右侧大脑中动脉栓塞建立短暂性脑缺血模型。在恢复再灌后48 h行MRI检查采集T2 FLAIR、常规DWI及AQP MRI图像,并行Western Blot和免疫荧光检测AQP4的表达量。结果实验组大鼠在T2 FLAIR上可见明显的高信号病灶,其T2信号的差值百分比显著高于假手术组(56.655±7.359和2.334±2.203,P<0.01);实验组病灶的ADC值差值百分比较假手术组显著降低(-24.491±1.924和-0.960±3.824,P<0.01);AQP ADC结果显示实验组的AQP ADC值的差值百分比较假手术组显著降低(-25.218±8.839和0.209±1.279,P<0.01)。Western Blot结果显示实验组患侧的AQP4蛋白表达量显著高于假手术组。将实验组单只大鼠的AQP ADC图像与其相应的免疫荧光结果对比显示,AQP ADC值高的区域,AQP4阳性染色减少,而AQP ADC值低的区域,AQP4阳性染色增多。结论 AQP MRI能够在体显示水通道蛋白的分布情况,对缺血性脑卒中的病程判断具有重要意义。

关键词(KeyWords)： 水通道蛋白磁共振分子成像;脑缺血;水通道蛋白;脑梗塞

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金面上项目(编号:81571838);; 福建省自然科学基金科技项目(编号:2014J01398);; 福建省卫生系统中青年骨干人才培养项目(编号:2014-ZQN-JC40);; 福建医科大学非直属附属医院科研发展专项基金(编号:FZS13021Y);; 福建省卫生厅青年项目(编号:2013-2-158)~~

作者(Author): 邢培秋;陈秋雁;吴富淋;彭晓澜;江敏;陈婷婷;魏鼎泰;

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DOI:

参考文献(References)：

• [1]Zhao ZS,Xiao ZK,Zhang Q,et al.Advance in aquaporins magnetic resonance imaging mechanism and application.Med Imaging,2013,10(11):42-45.赵周社,肖智魁,张泉,等.水通道蛋白磁共振分子成像机制和应用进展.医学影像,2013,10(11):42-45.
• [2]Lu H,Sun SQ.A correlative study between AQP4 expression and the manifestation of DWI after the acute ischemic brain edema in rats.Chin Med J(Engl),2003,116(7):1063-1069.
• [3]Kleindienst A,Fazzina G,Amorini AM,et al.Modulation of AQP4expression by the protein kinase C activator,phorbol myristate acetate,decreases ischemia-induced brain edema.Acta Neurochir Suppl,2006,96:393-397.
• [4]Cai SN,Zhu SM.The effects of ketamine pretreated on cerebral edema and AQP4 expression after transient focal cerebral ischemia/reperfusion in rats.Zhonghua Yi Xue Za Zhi,2010,90(23):1648-1651.
• [5]Qi LL,Fang SH,Shi WZ,et al.Cys LT2 receptor-mediated AQP4upregulation is involved in ischemic-like injury through activation of ERK and p38 MAPK in rat astrocytes,Life Sci,2011,88(1-2):50-56.
• [6]Chen QY,Wu FL,Miao SW,et al.Research on the mechanism of remote limb ischemic preconditioning prior to the lesion appearance at DWI in stroke.J Apoplexy Nervous Dis,2014,31(3):247-250.陈秋雁,吴富淋,缪绍维.肢体远端缺血预处理延缓脑卒中DWI上病灶出现的机制研究.中风与神经疾病杂志,2014,31(3):247-250.
• [7]Badaut J,Ashwal S,Obenans A.Aquaporins in cerebrovascular disease:a target for treatment of brain edema.Cerebrovas Dis,2011,3l(6):521-531.
• [8]Grinberg F,Farrher E,Ciobanu L,et al.Non-gaussian diffusion imaging for enhanced contrast of brain tissue affected by ischemic stroke.PLo S One,2014,9(2):e89225.
• [9]Obenaus A,Ashwal S.Magnetic resonance imaging in cerebral ischemia:focus on neonates.Neuropharmacology,2008,55(3):271-280.
• [10]Zhu JJ,Zhuo CJ,Qin W.Performances of diffusion kurtosis imaging and diffusion tensor imaging in detecting white matter abnormality in schizophrenia.Neuroimage Clin,2015,7:170-176.
• [11]Wei DT,Chen QY,Wu FL,et al.MR DWI research of the inhibition of limb remote ischemic preconditioning(LRP)on stroke.J Apoplexy Nervous Dis,2012,29(8):691-694.魏鼎泰,陈秋雁,吴富淋,等.肢体远端缺血预处理对抑制缺血性脑卒中的MR DWI研究.中风与神经疾病杂志,2012,29(8):691-694.
• [12]Lin YH,Sun XL,Cheng Y,et al.Research of aquaporin molecular imaging.Radiol Practice,2015,30(6):622-625.林艳红,孙夕林,程雁,等.水通道蛋白分子成像研究.放射学实践,2015,30(6):622-625.
• [13]Agre P,Sasaki S,Chrispeels MJ.Aquaporins a family of water channel proteinsⅢ.Am J Physiol Renal Physial,1993,3(265):F461.
• [14]Benga G.Birth of water channel proteins-the aquaporins.Cell Bio Int,2003,9(27):701-709.
• [15]Zelenina M.Regulation of brain aquaporins.Neurochem Int,2010,57(4):468-488.
• [16]Ishibashi K,Hara S,Kondo S.Aquaporin water channels in mammals.Clin Exp Nephrol,2009,13(2):107-117.
• [17]Lei Y,Ding Li,Ren LX,et al.Carotid atherosclerotic plaque composition analysis and classifi cation with 3.0 T MRI.Chin J Magn Reson Imaging,2015,6(6):430-436.雷云,丁里,任丽香,等.3.0 T MRI对颈动脉粥样硬化斑块成分分析及分型的研究.磁共振成像,2015,6(6):430-436.
• [18]Li HY.MRI appearances of carotid vulnerable plaques and prediction of the risk of ischemic stroke.Chin J Magn Reson Imaging,2016,7(1):16-19.李红英.颈动脉易损斑块MRI表现与缺血性脑卒中的风险预测.磁共振成像,2016,7(1):16-19.
• [19]Li JH,Li QJ,Yu B,et al.DWI-MRI multi b value of aquaporin molecular imaging mechanism and method research.J Chin Clin Med Imaging,2014,25(3):186-189.李佳慧,李秋菊,于兵,等.DWI-MRI多b值水通道蛋白分子成像机理和方法学研究.中国临床医学影像杂志,2014,25(3):186-189.
• [20]Li QJ,Li JH,Zhao ZS,et al.Assessment of aquaporins function of early-stage fibrosis using multi-b diffusion weighted magnetic resonance imaging.J Chin Clin Med Imaging,2014,25(10):719-723.李秋菊,李佳慧,赵周社,等.DWI多b值水通道蛋白分子成像在肝脏纤维化早期诊断的价值.中国临床医学影像杂志,2014,25(10):719-723.
• [21]Bai Y,Zhao ZS,Shi DP,et al.Aquaporin imaging technology and its application in glioma.Funct Mol Med Imaging(Elect Ed),2015,4(2):672-676.白岩,赵周社,史大鹏,等.水通道蛋白成像技术及其在脑胶质瘤中的应用进展.功能与分子医学影像学(电子版),2015,4(2):672-676.
• [22]Ling XY,Zhang ZP,Zhao ZS,et al.Investigation of apparent diffusion coefficient from ultra-high b-values in Parkinson's disease.Eur Radiol,2015,9(25):2593-2600.
• [23]Steiner E,Enzmann GU,Lin S,et al.Loss of astrocyte polarization upon transient focal brain ischemia as a possible mechanism to counteract early edema formation.Glia,2012,60(11):1646-1659.
• [24]Zeng XN,Xie LL,Liang R,et al.AQP4 knockout aggravates ischemia/reperfusion injury in mice.CNS Neurosci Ther,2012,18(5):388-394.
• [25]Papadopoulos MC,Manley GT,Krishna S,et al.Aquaporin-4facilitates reabsorption of excess fluid in vasogenic brain edema.FASEB J,2004,18(11):1291-1293.
• [26]Ribeiro Mde C,Hirt L,Bogousslavsky J,et al.Time course of aquaporin expression after transient focal cerebral ischemia in mice.J Neurosci Res,2006,83(7):1231-1240.
• [27]Vella J,Zammit C,Di Giovanni G,et al.The central role of aquaporins in the pathophysiology of ischemic stroke.Front Cell Neurosci,2015,9:108.