胡磊;查云飞;林苑;邢栋;龚威;王克军;闫力永;王娇;

• 1:武汉大学人民医院放射科

摘要(Abstract)：

目的探索定量非对称回波的最小二乘估算法迭代水脂分离序列(iterative decomposition of water and fat with echo asymmetry and least-squares estimation quantitation sequence,IDEAL-IQ)定量评估兔糖尿病模型椎体骨髓脂肪含量的可行性。材料与方法12只3月龄新西兰雄性大白兔随机分为糖尿病组(n=6)和对照组(n=6)。用四氧嘧啶对糖尿病组造模,在造模成功后各时间点(0、4、8、12、16周),对糖尿病组及对照组行腰椎矢状位T1WI、T2WI、IDEAL-IQ检查。在IDEAL-IQ脂肪分数图像测量兴趣区内脂肪含量比值;在16周处死糖尿病组和对照组的大白兔,取腰3⁷椎体做HE染色脂肪细胞计数;对实验组和对照组兔各时间点的脂肪含量比值采用重复测量的方差分析;对16周实验组和对照组IDEAL-IQ测量腰椎体脂肪比参数与HE切片镜下脂肪细胞计数行Pearson相关分析。结果16周实验组兔腰椎常规矢状位T1WI和T2WI显示诸腰椎椎体信号轻度增高。常规HE染色显示,16周糖尿病组兔椎体终板下骨髓腔脂肪含量较对照组明显增多,骨髓细胞明显减少。糖尿病组各时间节点椎体骨髓的脂肪含量比值差异存在统计学意义(F=50.387,P<0.01),对照组各时间点脂肪含量比值差异不存在统计学意义(F=1.477,P>0.05);16周实验组与对照组的椎体骨髓脂肪含量比值(FF%)差异有统计学意义(t=-10.726,P<0.01);IDEAL-IQ测量椎体脂肪含量比与脂肪细胞计数高度正相关关系(r=0.925,P<0.05)。结论 IDEALIQ定量评估兔糖尿病模型椎体骨髓脂肪含量是可行的。.

关键词(KeyWords)： 糖尿病,实验性;骨髓;脂肪定量;磁共振成像;实验动物科学;兔

Abstract:

Keywords:

基金项目(Foundation): 湖北省自然科学基金项目(编号:2013CFB242);; 湖北省卫生厅科研资助项目(编号:JX6B68)

作者(Authors): 胡磊;查云飞;林苑;邢栋;龚威;王克军;闫力永;王娇;

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