张双;信瑞强;石逸杰;李艳翠;彭如臣;

• 1:首都医科大学附属北京潞河医院放射科

摘要(Abstract)：

目的 利用生物信息学技术对骨关节炎(osteoarthritis,OA)差异表达基因进行识别和功能富集分析，并通过临床数据研究P53对基于MRI的OA间隙狭窄程度的影响。材料与方法 下载微阵列数据(GSE55235)，利用GEO2R鉴别OA患者与正常滑膜样本间的差异表达基因(differentially expressed genes,DEGs)。利用基因本体论(gene ontology,GO)和京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)对差异表达基因进行基因富集分析，进一步构建蛋白—蛋白互作(protein-protein interaction,PPI)网络分析，识别重要模块，鉴定核心基因。选取50例OA间隙狭窄患者，利用MRI检测其骨关节情况，分析其临床资料，运用Pearson卡方检验、Spearman相关性分析和多因素Logistic回归进行统计学分析。结果 对照组与OA组滑膜组织之间存在差异。GSE55235的分析结果识别出91个差异表达基因。DEGs集富集分析全面概述了OA的一些主要病理生理机制：细胞对过氧化氢的反应、P53信号通路。鉴定出了4个关键基因：MMP1、CXCL8、VEGFA和JUN。与正常组对比，MMP1在OA组中高表达，CXCL8、VEGFA和JUN在OA组中低表达。Pearson卡方检验、Spearman相关性分析和多因素Logistic回归结果显示P53与OA间隙狭窄程度显著相关(OR=0.112,95%CI:0.025～0.495,P=0.004)，而性别、年龄、MMP1、VEGFA、CXCL8与OA间隙狭窄程度无显著相关性(P值均大于0.05)。结论 基于MRI结果的基础上，判断细胞对过氧化氢的反应、P53信号通路能为OA的诊断和治疗提供新的研究靶点。P53在OA患者中低表达,可能作为其潜在治疗靶点。MRI也有利于OA患者早期的诊断与治疗，降低OA发病率。

关键词(KeyWords)： 磁共振成像;骨关节炎;生物信息学;差异表达基因;富集分析;P53信号通路

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 张双;信瑞强;石逸杰;李艳翠;彭如臣;

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